reactive oxygen species ros production  (Novus Biologicals)

Journal: PLOS ONE

Article Title: Clinical outcomes and anti-inflammatory mechanisms predict maximum heart rate improvement after physical activity training in individuals with psychiatric disorders and comorbid obesity

doi: 10.1371/journal.pone.0313759

Figure Lengend Snippet: MHR: maximum heart rate, HRSD: Hamilton rating scale for depression, PANSS-P: positive and negative syndrome scale (positive subscale), PANSS-G: positive and negative syndrome scale (general subscale), IL-10: interleukin 10, TNF-α: tumor necrosis factor alpha, ROS: reactive oxygen species; ΔΨm: mitochondrial membrane potential, VCAM: vascular cellular adhesion molecule, LEPMN: leukocyte-endothelium adhesion polymorphonuclear, RVPMN: rolling velocity polymorphonuclear cells, PSEL: P-selectin; MPO: myeloperoxidase.

Article Snippet: In total, 100 000 cells were plated in each well of 96-well plates and incubated for 30 min at 37°C with the corresponding fluorochromes, as follows: dichlorofluorescein diacetate to measure reactive oxygen species (ROS) production (485 nm excitation, 535 nm emission), MitoSOX to measure mitochondrial ROS (mROS) (510 nm excitation, 580 nm emission), tetramethylrodamin methyl ester to assess mitochondrial membrane potential (ΔΨm) (552 nm excitation, 574 nm emission), superoxide dismutase (SOD) (total SOD/T-SOD Activity Assay Kit–Colorimetric—from NOVUS), and 5-chloromethylfluorescein diacetate to measure intracellular glutathione (GSH) (492 nm excitation, 517 nm emission).

Techniques: Membrane